|
KMID : 0603820090150040319
|
|
Journal of Experimental & Biomedical Science
2009 Volume.15 No. 4 p.319 ~ p.326
|
|
|
Characteristics of Purinergic Receptor Expressed in 3T3-L1 Preadipocytes
|
|
Lee Hyung-Joo
Kong In-Deok
Kim Min-Jeong
Baik Joon-Heum
Kim Na-Hyun
|
|
|
Abstract
|
|
|
|
Extracellular ATP elicits diverse physiological effects by binding to the G-protein-coupled P2Y receptors on the plasma membrane. In addition to the short-term effects of extracellular nucleotides on cell functions, there is evidence that such purinergic signalling can have long-term effects on cell proliferation, differentiation and death. The 3T3-L1 cell line derived from mouse embryo is a well-established and commonly utilized in vitro model for adipocytes differentiation and function. However, the distributions and roles of P2Y subtypes are still unknown in the preadipocyte. In this study, we identified the distributions and roles of P2Y subtypes in preadipocyte using Ca²? imaging and realtime PCR. ATP increased the [Ca²?]i in a concentration-dependent manner. ATP increased Ca²? in absence and/or presence of extracellular Ca²?. Suramin, non-selective P2Y blocker, largely blocked the ATP-induced Ca²? response. U73122, a PLC inhibitor, completely inhibited Ca²? mobilization in 3T3-L1 cells. The mRNA expression by realtime PCR of P2Y subtypes was P2Y₂:P2Y?:P2Y?=1.0:12.5:0.3. In conclusion, we showed that P2Y? receptor is a dominant purinergic receptor in preadipocytes, and multiple P2Y receptors could involve in differentiation and migration via regulating of intracellular calcium concentration.
|
|
|
KEYWORD
|
|
|
Preadipocyte, P2Y?receptor, Intracellular calcium
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|